|
KMID : 0379119850130020075
|
|
Korean Journal of Mycology
1985 Volume.13 No. 2 p.75 ~ p.78
|
|
|
Isolation of Auxotrophic Mutants from Protoplasts of Pleurotus ostreatus and Pleurotus florida
|
|
|
|
|
Abstract
|
|
|
|
Mutagenesis of protoplast could serve a great potential tool for improvement of strains and genetics in higher fungi. For the isolation of auxotrophic mutants from protoplasts of Pleurotus ostreatus and Pleurotus florida, viability levels of ultraviolet lights were determined. Seven auxotrophs were obtained from protoplasts irradiating UV to give 0.83∼15% survival. The mutants showed a single requirement for each of Arg, Ribo-1, Ribo-2 or Phen for growth. Some of them showed two or three kinds of requirements, Gly Ser, Ade Hypo or Ala Orn Tryp for growth.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|